📚 Seth MB


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Light Microscopy

Last updated May 26, 2023 Edit

Maximum resolution: 200nm

Any objects closer than 200nm will appear as one object under the best light microscope.

2 objects can only be seen if light is able to pass between them.

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# Sectioning

When studying a section, structures may be seen differently depending on how the section was taken relative to the structure. So a mitochondria may have been cut vertically whilst you are looking for one cut horizontally. It is important to account for this.

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# Microscope Terminology

Artifacts are damaged specimens caused by mechanical or chemical damage during slide preparation.

Here are a few examples of artifacts that could be misidentified as different objects.

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