📚 Seth MB

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Types of Microscope

Last updated May 26, 2023 Edit

# Super Resolved Fluorescence Microscope

Advantages

  • Stains that flouresce reveal different structures
  • Good for viewing DNA + molecules

Disadvantages

  • Some dyes interfere with cell parts

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# Laser Scanning (Confocal) Microscope

Advantages

  • Non-invasive clinical diagnosis
  • 3D image overlays

Disadvantages

  • Resolution limited by wavelength of light
  • Dyes can interfere with cell function

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# Atomic Force Microscope

Advantages

  • Normal cell conditions
  • Living systems
  • Drug interactions

Disadvantages

  • Can’t see inside cells, surface only

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# Transmission Electron Microscope (TEM)

Advantages

  • Seeing ultra structure

Disadvantages

  • Expensive
  • Large
  • Prone to artifacts
  • Samples will be killed

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# Scanning Electron Microscope (SEM)

Advantages

  • 3D image
  • Good for organism appearance

Disadvantages

  • Very large
  • Expensive
  • Samples dead
  • Salt stains are dangerous
  • Requires training

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Cells