Enzymes
# What are enzymes?
- Biological catalysts
- Inside cells
- protein/lipid synthesis
- intercellular
- Outside cells
- digestion
- extracellular
- Globular proteins
- Generally soluble in water
- Functions
- Speed up a reaction
- Help build a larger molecule
- Help breakdown a large molecule into multiple smaller ones
- Speed up a reaction
# Activation energy
- Amount of energy which needs to be supplied for a reaction to proceed.
- Enzymes reduce activation energy requirements (due to their specific shape)
# Induced fit hypothesis
Substrate molecules collide with enzyme’s active site
Enzyme molecule changes shape slightly. Active site fits more closely around substrate.
Enzyme-substrate complex (ESC) is formed.
Substrate destabilised
Product produced
Enzyme-product formed
Product leaves enzyme active site
All enzymes are globular proteins
- More beta pleated sheets than alpha helix
Many hydrophilic R groups