Super Resolved Fluorescence Microscope

  • Uses light
  • Very high resolution (0.2nm)
  • x1500 magnification

Advantages

  • Stains that flouresce reveal different structures
  • Good for viewing DNA + molecules

Disadvantages

  • Some dyes interfere with cell parts

Laser Scanning (Confocal) Microscope

  • Uses light
  • 500nm resolution
  • x1000 magnification
  • Parts are labelled with a fluorescent dye.

Advantages

  • Non-invasive clinical diagnosis
  • 3D image overlays

Disadvantages

  • Resolution limited by wavelength of light
  • Dyes can interfere with cell function

Atomic Force Microscope

  • “Feels” surface of specimen
  • Uses light via a laser diode
  • 0.1nm resolution
  • x100 000 000 magnification

Advantages

  • Normal cell conditions
  • Living systems
  • Drug interactions

Disadvantages

  • Can’t see inside cells, surface only

Transmission Electron Microscope (TEM)

  • Magnification up to x2000000
  • 2D greyscale image
  • Resolution of 0.5nm

Advantages

  • Seeing ultra structure

Disadvantages

  • Expensive
  • Large
  • Prone to artifacts
  • Samples will be killed

Scanning Electron Microscope (SEM)

  • Magnification between x15 and x200000
  • Resolution between 3nm and 10nm
  • Sample kept in vacuum

Advantages

  • 3D image
  • Good for organism appearance

Disadvantages

  • Very large
  • Expensive
  • Samples dead
  • Salt stains are dangerous
  • Requires training

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